The Effect of a Mutation in the Shine-Dalgarno Sequence Preceding the Rop Gene on the Level of Rop Protein Produced
Palsrok, Kelli L.
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The exact mechanism by which plasmid replication is controlled is not yet known. The key elements in plasmid copy number control have been identified as RNA I, RNA II and rop. The most widely accepted model hypothesizes that in order for plasmid replication to occur, RNA II must form a stable hybrid with the plasmid DNA. By binding to the RNA II preprimer, RNA I is thought to reduce the initiation of plasmid replication by inhibiting formation of a stable RNA II-DNA hybrid. The rop protein is thought to mediate the formation of the RNA I-RNA II hybrid. It has previously been determined that if the rop gene is deleted, copy number increases two- to five-fold . In this paper we examine the effect of a single base change mutation in the Shine-Dalgarno sequence of the rap gene on the level of rap produced through the use of a rop-lacZ gene fusion.