The Production of Monoclonal Antibodies against the Calcium Dependent Cell Adhesion Molecule and N-Acetylgalactosaminyltransferase Found on the Surface of Embryonic Chick Neural Retina Cells
Abstract
Embryonic chick neural retina cells have at their surface a
complex of molecules involved in the cell to cell adhesion
process. Two of the most important components of this complex
are the calcium dependent neural cell adhesion molecule (NcaICAM)
and an enzyme, N-acetylgalactosaminyltransferase (retina galNAc
transferase). Both molecules can be isolated from retina cell
membranes with Triton X-l00 detergent and used as immunogens.
This paper is a report on a cell fusion and the associated
processes used to generate monoclonal antibodies against both
NcalCAM and retina galNAc transferase to aid in the further
analysis of these molecules. It also involves an attempt to
integrate a new fusion protocol involving the use of Robertsonian
(8.12) 5Bnr mice and the FOX-NY myeloma cell line into the
repertoire of techniques being used in the laboratory of Dr. Jack
Lilien, University of Wisconsin at Madison, to explore the many
facets of cell adhesion. Preliminary results indicate that the
hybridomas produced during the cell fusion might be producing
antibodies against both NcalCAM and retina galNAc transferase
and, if so, the use of Robertsonian mice and the FOX-NY cell line
would prove to be a valuable technique for the lab.