Possible Coordinate Regulation of Apolipoproteins B-100 (LDL) and A-1 (HDL) Via the LDL Receptor Pathway
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Authors
Vaishampayan, Nitin G.
Issue Date
1987
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
One of the major factors contributing to coronary
heart disease is high plasma levels of cholesterol.
Studies of hypercholesterolemia have focused on the role
of lipoprotein particles: Low Density Lipoproteins (LDL),
which contain the apolipoprotein B-100, appear to play a
causal role in the development of cholesterol plaques;
High Density Lipoproteins (HDL), which contain the
apolipoprotein A-I, appear to prevent this process. In
this study, the levels of apolipoprotein B-100 and
apolipoprotein A-I messenger RNA levels were measured
using specific cDNA probes. Hepatoma G2 cells were grown
in media containing lipoprotein deficient serum to which
low density lipoprotein was added to some flasks. The RNA
was extracted and the levels of the specific mRNAs
determined by Northern Blot or Dot Blot hybridizations.
The results indicated that the level of apolipoprotein
B-100 mRNA was lower in cells grown in the presence of LDL
than cells grown in lipoprotein deficient serum alone.
The levels of apolipoprotein A-I mRNA, on the other hand,
were variable and could not be related to the presence of
LDL. While the results suggest that exogenous LDL can
repress apolipoprotein B-100 formation, further studies
would be required to determine whether LDL and HDL
biosynthesis are coordinately regulated.
Description
vi, 36 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.