Diagnostic DNA Hybridizations for the Presence of Cytomegalovirus
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Authors
Boyd, James
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
As a better understanding of the prevalence and
pathogenicity of Cytomegalovirus (CMV) has been gained
over recent years, the need for a fast, accurate, and
inexpensive detection method has become increasingly
important. The most promising technique is the use of
a CMV-derived DNA "probe" to detect the viral DNA.
The probe would be most functional if it could accurately
detect CMV in urine and blood samples. Much of the work
on the urine probe has been completed. For the blood
probe, the DNA from Red Cross donor bloods was isolated
and stored for testing. This report focuses on what
is perhaps the first attempt to detect CMV in blood using
a DNA probe.
Two probes from the Towne strain of CMV were used.
One contained the complete genome, and the other
consisted of the Xba I restriction fragment "E", which
has been reported to show little homology with human
DNA. Unfortunately, this first attempt proved to be
inconclusive.
Description
iv, 28 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.