Diagnostic DNA Hybridizations for the Presence of Cytomegalovirus
As a better understanding of the prevalence and pathogenicity of Cytomegalovirus (CMV) has been gained over recent years, the need for a fast, accurate, and inexpensive detection method has become increasingly important. The most promising technique is the use of a CMV-derived DNA "probe" to detect the viral DNA. The probe would be most functional if it could accurately detect CMV in urine and blood samples. Much of the work on the urine probe has been completed. For the blood probe, the DNA from Red Cross donor bloods was isolated and stored for testing. This report focuses on what is perhaps the first attempt to detect CMV in blood using a DNA probe. Two probes from the Towne strain of CMV were used. One contained the complete genome, and the other consisted of the Xba I restriction fragment "E", which has been reported to show little homology with human DNA. Unfortunately, this first attempt proved to be inconclusive.