Preliminary Studies on the Physiological Effect of a Porcine Ileal Polypeptide on Stomach Contractility in Canine Antrum and Rat Fundus
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Authors
Koziol, Wladyslaw W.
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
A porcine ileal polypeptide, PIP, has been isolated from porcine ileal
mucosa by precipitation with (NH4)2S04, followed by fractionation by gel
filtration. The protein has a molecular weight of 13,000 daltons as determined
by SDS electrophoresis, and was found to contain 124 residues as determined
by amino acid composition. PIP has been shown to inhibit gluconeogenesis in
isolated rat hepatocytes. In isolated parietal cells of the canine stomach, PIP has been
found to stimulate acid secretion at hormonal levels. More recently, Wider has studied the effect PIP has on acid secretion in the
rat stomach in vivo. Preliminary results suggest that PIP stimulates acid
secretion.
This paper describes the results of a preliminary study on the effect of
PIP on stomach smooth muscle contractility in canine antrum and rat fundus
in vitro. Muscle strips were studies in vitro using established organ bath
techniques. Although definitive conclusions can not be reached, the results
suggest that PIP may stimulate relaxation in the rat fundus longitudinal muscle
and slightly stimulate contractility in the canine antrum circular muscle.
The results from this line of inquiry merit further investigation on PIP's
role in the gastrointestinal tract.
Description
vi, 30 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.