In Vitro Studies on the Rat Superior Cervical Ganglion and Dorsal Root Ganglion
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Authors
Ebell, Mark H.
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The in vitro behavior of Schwann cells isolated from
rat embryonic day 15 superior cervical ganglia (E15 SCG)
was studied by the techniques of autoradiography and immunocytochemistry.
It was determined that a population of Schwann
cells isolated without the use of antimitotic drugs is quiescent
(non-proliferative) in the absence of neurites. These
quiescent Schwann cells did not respond to fibroblast growth
factor as a mitogen. A new cell type was observed in primary
neuritic outgrowths of E15 SCG. These cells were proliferating,
and did not stain for antibody to fibronectin (characteristic
of fibroblasts) or laminin (characteristic of Schwann cells).
These cells may represent an early developmental stage of
Schwann cells. a so-called "Schwann-blast". Studies on E14
and E20 rat dorsal root ganglia in vitro indicated that the
type of collagen substratum can affect both the nature of the
non-neuronal cell populations (fibroblasts and Schwann cells)
and their migration in the neuritic outgrowth. Evidence
was also acquired to support the view that fibroblasts can
significantly affect the morphology of the neuritic outgrowth.
Description
vi, 37 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.