In Vitro Studies on the Rat Superior Cervical Ganglion and Dorsal Root Ganglion
The in vitro behavior of Schwann cells isolated from rat embryonic day 15 superior cervical ganglia (E15 SCG) was studied by the techniques of autoradiography and immunocytochemistry. It was determined that a population of Schwann cells isolated without the use of antimitotic drugs is quiescent (non-proliferative) in the absence of neurites. These quiescent Schwann cells did not respond to fibroblast growth factor as a mitogen. A new cell type was observed in primary neuritic outgrowths of E15 SCG. These cells were proliferating, and did not stain for antibody to fibronectin (characteristic of fibroblasts) or laminin (characteristic of Schwann cells). These cells may represent an early developmental stage of Schwann cells. a so-called "Schwann-blast". Studies on E14 and E20 rat dorsal root ganglia in vitro indicated that the type of collagen substratum can affect both the nature of the non-neuronal cell populations (fibroblasts and Schwann cells) and their migration in the neuritic outgrowth. Evidence was also acquired to support the view that fibroblasts can significantly affect the morphology of the neuritic outgrowth.
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