Isolation and Partial Restriction Mapping of a Fifth Histone Gene Clone in Artemia salina
Loading...
Authors
David, Andrew J.
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Histone gene expression is coordinated with DNA replication
in newly hatched larvae of the brine shrimp Artemia salina. A
Charon 4A library or Artemia genomic DNA fragments was screened for histone genes using pPS 101, a 3.4 kb
subclone from ~BSh4, an Artemia histone gene recombinant previously
isolated from the same library. As a result of this screening two
different recombinants were isolated. Upon digestion with the
restriction endonuclease EcoR1 one recombinant was determined to
be a reconstruction of Charon 4A insert pieces, whereas the second
recombinant, ABSh14D, with an insert length of 10.5 kb, gave a
digestion pattern different from any previously isolated Artemia
histone gene recombinant. Further probing of EcoR1 digested
ABSh14D DNA fragments using subclones, pPS 101, pPS 104, pPS 107
and pPS 111 from ~Sh4 gave positive hybridization results for
two bands of sizes 7.5 kb and 1.86 kb. Hybridization with
pP8 107, a subclone known to contain regions homologous to
Drosophila 5S rRNA, also gave a positive hybridization signal
for the 7.5 kb fragment indicating there may be regions in
~Sh14D that are, or resemble, 5S rRNA genes.
Description
vi, 41 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.