Effects of Various Hepatotoxins and Possible Protective Agents on In Vitro Hepatocytes
Weston, Kathleen A.
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The liver is the site of damage by many toxins, therefore protection of the liver from toxic damage is of interest to researchers. In the present study, hepatotoxins and possible protective agents were examined in vitro. Monolayer primary cultures of isolated rat hepatocytes were used in the study. The hepatocytes were cultured in a medium designed to maintain the intracellular cytochrome P450 concentration. Cells were challenged with a saturated carbon tetrachloride solution and varying concentrations of 16,16-dimethyl PGE2. Protection was measured by an increase in cell viability, as determined by trypan blue exclusion. In addition, hepatocytes were challenged with other hepatotoxins, including CUCl 2 , the calcium ionophore A23l87, and hypertonic and hypotonic buffer solutions. possible protectants examined with these challenges included ethanol, methanol and glucose. Data indicates that in the incubation system used, 16,16-dimethyl PGE2 was not able to protect the hepatocytes against CCl4-induced damage. Further, ethanol (17 moM) was found to protect against copper damage to the cells, but not against damage by the other toxins studied.