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dc.contributor.advisorLoesche, Walter J.
dc.contributor.authorWilson, Ann C.
dc.descriptionviii, 38 p.en_US
dc.description.abstractVarious strains of black-pigmented Bacteroides were screened for antagonistic interactions by using a spiral plating technique. The initial screening procedure included cross combinations of Bacteroides gingivalis , Bacteroides melaninogenicus ssp. intermedius and Bacteroides melaninogenicus ssp. melaninogenicus. Pure cultures grown on an enriched trypticase soy agar (ETSA) medium were transferred to reduced transport fluid. Samples were dispersed, serially diluted and plated on ETSA under anaerobic conditions. Each plate was inoculated with a test strain deposited at a variable amount and an indicator strain deposited at a constant amount. After anaerobic incubation, plates were examined microscopically for inhibition. Antagonististic interactions were found only between strains of B. gingivalis and B. mel. ssp. intermedius. A second antagonism screening included 36 combinations of B. gingivalis and B. mel. ssp. intermedius. Each test consisted of four plates - two control and two double-inoculated plates. After anaerobic incubation, colonies of the control and indicator strains were counted and compared. Inhibition of B. mel. ssp. intermedius by B. gingivalis was found in 16 of the 36 combinations. The 16 cases of positive inhibition were duplicated on two different media-buffered ETSA and MM10 without sucrose. Selected antagonistic combinations were examined by three additional techniques including cross streaking, filtering of end products and diffusion. Positive inhibition of B. mel. ssp. intermedius by B. gingivalis was clearly detected in the first two of these tests.en_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleSpiral Plating Technique Applied to the Detection of Antagonism between Two Oral Species of Black-Pigmented Bacteroidesen_US
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  • Biology Senior Individualized Projects [1520]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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