Degradation of Striatal Dopamine in Tissue Extracts Taken from C3He/J Mice
Thall, Aron D.
MetadataShow full item record
The project was to develop a procedure which would enable a rapid and efficient measurement of the purity of dopamine. The procedure was designed to determine how much of the dopamine originally present in a tissue homogenate buffer solution remained as a function of time and was specifically designed for a receptor binding assay involving dopamine as a ligand. It involved the stabilization of tritiated dopamine by acetylation and its isolation by extraction. The purity of tritiated dopamine was found to decrease by 2% during the duration of the receptor binding assay. Ascorbic acid. a potent antioxidant. had no effect on the purity decrease of dopamine in the homogenate.