In Vitro Toxicity in Primary Adult Rat Hepatocyte Cultures
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Authors
Stout, Cheryl L.
Issue Date
1982
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Primary adult rat hepatocyte monolayer cultures were used to
study the toxic effects of methylmethanesulfonate (MMS) and
lysolecithin. Three methods were used to evaluate the possible
cytotoxic effects of the two drugs: release of two intracellular
enzymes, lactate dehydrogenase (LDH) and serum glutamic oxaloacetate
transaminase (SGOT); trypan blue exclusion; and scanning
electron microscopy. Cultures were treated with 50, 100, and 200
µg/ml MMS and 0.5 ml aliquots of medium were removed after 2 and 6
hours of incubation for the enzyme analysis. Cultures were
treated with lysolecithin at concentrations at 1.0, 15, 50, and
200 µg/ml and 0.5 ml aliquots of medium were withdrawn at 1, 3, 6,
and 24 hours after treatment. Trypan blue exclusion and scanning
electron microscopy were performed after all aliquots of medium
had been removed for enzyme assay. A time- and dose-dependent
cytotoxic effect of both drugs was demonstrated. The results
indicate that a combination of methods to evaluate cytotoxicity is
advantageous and that the cell culture system is an excellent
method for screening drugs for toxicity.
Description
v, 39 p.
Citation
Publisher
Kalamazoo College
License
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