The Effects of Endotoxin on Kupffer Cells
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Authors
Diamantoni, George A.
Issue Date
1984
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Kupffer cells (macrophages) play an important function
in the capturing of particulate material arriving at the
liver via the portal circulation. Exhaustion of the
phagocytic capacity of these cells increases the lethality
of endotoxin. In order to evaluate the possible role of the
Kupffer cell in endotoxic (lipopolysaccharide (LPS))-induced
shock, a technique has been developed for the isolation and
maintenance in culture of rat Kupffer cells.
Characterization of the resultant cell population by
morphology, peroxidase staining,and phagocytosis of latex
particles confirms the presence of a pure population of
Kupffer cells without outgrowth of other cell types for
several days in culture. In vivo, treatment with LPS
produces an apparent direct cellular toxicity. There,
investigations show that the combined effect of toxicity,
selective stimulation, and release of mediators in
LPS-stimulated macrophages may play a central role in the
endotoxemic shock syndrome.
Description
v, 43 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.