The Effects of Endotoxin on Kupffer Cells

Loading...
Thumbnail Image
Authors
Diamantoni, George A.
Issue Date
1984
Type
Thesis
Language
en_US
Keywords
Research Projects
Organizational Units
Journal Issue
Alternative Title
Abstract
Kupffer cells (macrophages) play an important function in the capturing of particulate material arriving at the liver via the portal circulation. Exhaustion of the phagocytic capacity of these cells increases the lethality of endotoxin. In order to evaluate the possible role of the Kupffer cell in endotoxic (lipopolysaccharide (LPS))-induced shock, a technique has been developed for the isolation and maintenance in culture of rat Kupffer cells. Characterization of the resultant cell population by morphology, peroxidase staining,and phagocytosis of latex particles confirms the presence of a pure population of Kupffer cells without outgrowth of other cell types for several days in culture. In vivo, treatment with LPS produces an apparent direct cellular toxicity. There, investigations show that the combined effect of toxicity, selective stimulation, and release of mediators in LPS-stimulated macrophages may play a central role in the endotoxemic shock syndrome.
Description
v, 43 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
Journal
Volume
Issue
PubMed ID
DOI
ISSN
EISSN