The Effects of Endotoxin on Kupffer Cells
Diamantoni, George A.
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Kupffer cells (macrophages) play an important function in the capturing of particulate material arriving at the liver via the portal circulation. Exhaustion of the phagocytic capacity of these cells increases the lethality of endotoxin. In order to evaluate the possible role of the Kupffer cell in endotoxic (lipopolysaccharide (LPS))-induced shock, a technique has been developed for the isolation and maintenance in culture of rat Kupffer cells. Characterization of the resultant cell population by morphology, peroxidase staining,and phagocytosis of latex particles confirms the presence of a pure population of Kupffer cells without outgrowth of other cell types for several days in culture. In vivo, treatment with LPS produces an apparent direct cellular toxicity. There, investigations show that the combined effect of toxicity, selective stimulation, and release of mediators in LPS-stimulated macrophages may play a central role in the endotoxemic shock syndrome.