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    A Comparison Involving a Wild-Type Strain of Pseudorabies Virus (Rice) and a Vaccine Strain of Pseudorabies (Norden) Using Methods of Restriction Enzyme Mapping and DNA Sequencing

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    Date
    1985
    Author
    Gierman, Todd M.
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    Abstract
    Pseudorabies virus (PrV), a member of the herpesvirus sub-family alphaherpesvirinae, causes the illness known as Aujezsky's disease. PrY is virulent in all mammals, with the exception of the higher primates, i.e the great apes and humans. The virus is responsible for an economic loss exceeding $100 million annually through the infection of livestock, the primary target animals of PrY. As the adult pig is capable of surviving infection, with the virus often going latent, it serves as the primary reservoir of Pr V. Several attenuated vaccine strains of PrY are currently being marketed in the United States and Europe, one of which, PrY Norden, contains a 3.5-Kb deletion in the Us portion of its genome, in comparison to the wild-type strain of PrY, Rice. It has been shown previously that this deletion is at least partially responsible for Norden's avirulence in pigs. Through the comparison of PrY Norden with the wild-type strain of PrY, Rice, this study has determined the precise location of this deletion using methods of restriction enzyme mapping and end-labeled DNA sequencing. The results generated in this study indicate that the genes which code for the viral envelope glycoproteins gX, gp50, and gp63 are not affected by the Us deletion, but that the gene which codes for the glycoprotein gI has been deleted, as well as a series of genes whose functions are unknown. The deletion begins in the region of BamH I 7, 77 bases downstream from the gp63 termination codon, and extends 5OO bases into the region of BamH I 12. In addition to the characterization of the Norden Us deletion, several restriction maps have been constructed using a series of restriction endonucleases which were largely untested on PrY DNA. The generation of such restriction maps has illustrated several regions of heterogeneity between the two strains, and should prove useful in future comparisons of the two strains of PrY.
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    http://hdl.handle.net/10920/22943
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    • Biology Senior Integrated Projects [1613]

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      Location, Cloning and Restriction Mapping the Thymidine Kinase Gene from Rice Strain Pseudorabies Virus 

      Koshy, Thomas I. (Kalamazoo College, 1984)
      The pseudorabies virus (PRV) i a porcine herpesvirus that lethally infects most mammals excluding the higher primates. While the virus may not be lethal to swine, it generates millions of dollars in economic losses annually. ...
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      Restriction Endonuclease Maps of the Pseudorabies Virus, Rice Strain, Genome 

      Long, Graham W. (1983)
      Pseudorabies virus (PRV) is a herpesvirus that attacks most mammals and has caused serious economic problems in the swine industry. Since available vaccines have not been efficacious in controlling this virus, more ...
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      Caracterization of the Processing of Glycoprotein High C of Pseudorabies Virus 

      Bennett, L. Michelle (Kalamazoo College, 1984)
      Pseudorabies virus (PRV) is anΑα-herpesvirus and molecularly similar to Herpes Simplex Virus (HSV). The virus may lethally infect all mammals with the exception of the higher primates. Although susceptible to infection, ...

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