The Vector Immortalization of a Normal Adult Lymphocyte Cell Line by Deae-Dextran Transfection
Des Rosiers, J. Gregory
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The purpose of this study was to determine if it would be possible to introduce a viral clone composed of the Early 1A gene from a human type 5 adenovirus ligated to the .9 kilobase Long Terminal Repeat (LTR) of the Human T-cell Leukemia Virus type II, and whether such a clone would allow for the immortalization of these cells. It was found through in situ nucleic acid hybridization that the clone did enter the cells. Yet, as viability was monitored, a continuously decreasing number of cells remained viable, indicating that the clone proved insufficient in creating a long standing, Interleukin II-free T-Iymphocyte cell line.