Two Dimensional Electrophoretic Analysis of the Relative Turnover Rates of Individual Subfornical Organ Proteins under Conditions of Water Deprivation and Salt Loading
Santer, Donna Marie
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Fluid balance is important for all living organisms, and the subfornical organ of the brain, a midline, circumventricular organ, has a well established has a role in the regulation of fluid balance in higher animals. For four days, three groups of rats were given an appropriate fluid diet (control, 2% NaCl, and water deprived), with all groups having free access to food. Rats were killed by decapitation and the SFO quickly dissected out, and incubated in medium containing 35S-methionine and 35S-cysteine for 6 hours. The proteins from these organs were then separated by two-dimensional electrophoresis. The gels were then stained, photographed, dried and exposed to autofluorographic film. The results show that relative turnover rate of 8 proteins were changed due to experimental manipulations.