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    The Dynamics of Anti-Lymphocyte Activity in Horses Hyperimmunized with Human Thymus Tissue

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    Date
    1985
    Author
    Zotovas, Andrea
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    Abstract
    Horse plasma was obtained at 30-day timed intervals from horses which were hyperimmunized with human thymocytes. Rosette Inhibition Titer assay of total immunoglobulin fractions from these samples indicated that the anti-thymocyte activity remained relatively constantly after initial increase at 30 days. Lymphoagglutination assay of the same samples indicated an increase in anti-thymocyte activity after 90 days, while lymphocytotoxicity decreased after 90 days. Purified lgT and IgG subfractions from three of these five samples were also assayed. Rosette Inhibition Titer, Lymphoagglutination and lymphocytotoxicity assay of the purified IgT and IgG subfractions did not indicate that a disproportionate amount of the anti-thymocyte activity of this hyperimmune equine immunoglobulin resides in IgT.
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    http://hdl.handle.net/10920/22927
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