Penetration, Mode of Action, and Metabolism of C14-Labelled Banamite in Boll Weevils
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Radiolabelled benzoyl chloride (2,4,6-trichlorophenyl) hydrazone was topically applied to boll weevils. A time study was made to investigate the rate of penetration, the mode of action g and the metabolism of the compound within the insect. At an approximate dosage of 0.5µg, 75% of the compound will penetrate the insect within 24 hours. The original compound, benzoic acid, -2(2,4,6- trichlorophenyl) hydrazide, and 2,4,6-trichloroaniline, all containing C14 , were detected within the insect and the latter two are probably metabolic products of the first. Tracing the location of the highest concentrations of radioactivity indicates that following penetration the compound is in a benzene-soluble state, it enters a water-soluble state, then settles in the solid integumentary structures of the insect. Radioactivity was also found in the feces and in volatile respiratory products. The mode of action considered here involves noncompetitive complexing of the Banamite with an essential enzyme. This consideration deserves further study.