Selected Aspects of Macrophage Biology
Simpson, Rosetta Loree
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This paper is first addressed to delayed hypersensitivity in vitro using rabbit alveolar macrophages, wh1ch belong to a group characterized by a relatively high mitotic rate and the ability to attack and spread readily on glass, rather than peritoneal macrophages of guinea pigs. Several experiments were initiated with rabbit lymphocytes and human peripheral leucocytes to determine the techniques involved in the cell migration of lymphocytes and peripheral leucocytes. Also, tissue cultures of alveolar macrophages were set up to observe the motility of the cells and to extend the life span of macrophages after having been collected from the lungs. Secondly, this paper describes the function of rabbit alveolar macrophages in rosette formation and rosette inhibition. It is known that human lymphocytes react through an immunospecific mechanism with Sheep Red Blood Cells (SRBC) causing 5 or 6 SRBC to adhere to lymphocytes, forming a rosette. The lymphocytes have receptors that recognize the antigen (SRBC). Rosette-inhibition means that the inhibitor coats the lymphocyte, thus inactivating it by covering their receptors which cannot pick up SRBC. It was of theoretical and practical interest to determine whether or not rabbit. alveolar macrophages formed rosettes in the SRBC system. The report describes further rosette formation and rosette inhibition with human lymphocytes and rabbit lymphocytes.