Quantity of Interferon Produced and Sensitivity to Protective Effect of Interferon in Different Mouse Strains
MetadataShow full item record
Mouse embryo fibroblast (MEF) cultures derived from NIH Swiss, C3H/Hen, C57Bl/6N, and Balb/c mice were studied for amounts of interferon produced after stimulation with several viruses. No significant differences between mouse strains were found when Newcastle disease virus was used. This result was compared with literature reporting on NDV-induced circulating interferon production in the same strains. Differences in the relative sensitivity to protective effect of pretreatment with interferon against Sindbis virus in MEF cultures derived from the different mouse strains were studied. The differences found in MEF cultures did not correspond with in vivo differences in relative protective effect of prophylactic treatment with interferon against Semliki Forest virus in the same mouse strains. These results indicate that MEF cultures do not reflect the genetic differences found in amounts of circulating interferon produced, or in sensitivity to interferon protection in vivo. The mechanisms and genetics causing the differences in sensitivity to interferon protection in vivo could be explored by methods using intact mice of inbred strains such those used in this study.
Showing items related by title, author, creator and subject.
A Study of the Interferon Assay and the Effect of interferon and Tunicamycin on Cell Proliferation and Interleukin Production Armbrustmacher, Paula Michelle (1988)Interferons (INF) are the body's first line of defense against viral infection. There are three groups of IFN which are induced and produced by different mechanisms and an assay has been developed to ascertain the amount ...
A Study of Cell-Surface Characteristics of Macrophage Hybridomas and How tthey are Affected by Activation of the Macrophage Hybridomas with Gamma-Interferon Ahmed, Eqbal (1986)A series of macrophage hybridomas were geerated by fusion of splenic adherent cells with macrophage P388Dl tumor cells. The production of such a series of macrophage clones is of great assistance in analyzing macrophage ...
The Effect of Listeria Monocytogenes Priming on the Gamma Interferon Response in Cytomegalovirus-Infected Mice Petersen, Holly (1982)Gamma interferon has antiviral, antitumor, and immunoregulatory properties. One current area of research in which the activity of interferon is being explored is that of recurrent herpesvirus infection. Various agents known ...