Hydrolysis of Ara-C Esters in Mouse and Human Body Fluids

Abstract

Enzymatic hydrolysis rates for 21 ara-C esters were determined using the Streptococcus faecalis assay for ara-C. Rates obtained in the two human fluids studied -- plasma and synovial fluid - correlated well. Mouse plasma results correlated with these two to a lesser extent. Attempts to relate these rates to in vitro nonenzymatic hydrolysis data and in vivo biological activity failed to produce any strong correlations.