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dc.contributor.advisorLevy, S. B.
dc.contributor.advisorEvans, David A., 1938-
dc.contributor.authorNorman, Paula A.
dc.description19 p.en_US
dc.description.abstractR factors were segregated into minicells of R+ Escherichia coli minicell-producing strains. In this study, was demonstrated the ability of these R factors to transfer themselves from minicells into normal cells and express an antibiotic marker of the donor in the recombinant by conjugation. Minicells were harvested as well as cells from the first differential centrifugation (original cells) and from the cell pellets of two sucrose gradients (sucrose cells). It was found that transfer of the R factor from minicell to normal cell was as efficient as normal cell-to-cell transfer and that both the requirements for and the kinetics of transfer may be very similar to those of normal cells.en_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleTransfer of Segregated R Factors from Escherichia coli Minicells to Normal Cellsen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1520]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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