Transfer of Segregated R Factors from Escherichia coli Minicells to Normal Cells
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Authors
Norman, Paula A.
Issue Date
1970
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
R factors were segregated into minicells of
R+ Escherichia coli minicell-producing strains. In
this study, was demonstrated the ability of these R
factors to transfer themselves from minicells into
normal cells and express an antibiotic marker of the
donor in the recombinant by conjugation. Minicells
were harvested as well as cells from the first differential
centrifugation (original cells) and from the
cell pellets of two sucrose gradients (sucrose cells).
It was found that transfer of the R factor from minicell
to normal cell was as efficient as normal cell-to-cell
transfer and that both the requirements for and the
kinetics of transfer may be very similar to those of
normal cells.
Description
19 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.