Tranfer of Triglyceride Between Lipoproteins by a Protein Complex Isolated from Lipid-Free Plasma
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Authors
Pickford, Laura A,
Issue Date
1980
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Human plasma was found to contain a lipid transfer
complex (LTC) in the density>1.21 g/ml infranatant which
facilitated the transfer of cholesteryl esters, phosphatidylcholine
and triglyceride between lipoproteins. Triglyceride
transfer occured between very low density lipoproteins (VLDL)
and low density lipoproteins (LDL), but there was no reciprcal
transfer of cholesteryl ester. Triglyceride transfer also
occured between VLDL and HDL and there was a reciprocal
transfer of cholesteryl ester. Parachloromercuryphenyl
sulfonate (PCMPS) inhibited triglyceride transfer from VLDL
to LDL and HDL, however it did not inhibit cholesteryl ester
transfer from HDL to VLDL. These data suggested that the
LTC contains two transfer proteins, one for triglyceride and
one for cholesteryl ester,or one protein with two different
active sites, one of which was inhibited by PCMPS. The effects
of triglyceride transfer between VLDL and LDL on the catabolism
of VLDL were tested in an in vitro assay containing bovine
milk lipoprotein lipase and albumin. The transfer of
triglyceride to LDL and HDL was accompanied by a decrease
in the rate of VLDL catabolism.
Description
32 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.