Germination and Growth of Some Ectomycorrhizal Basidiomycetes in Culture
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Authors
Losinger, Willard Carl
Issue Date
1980
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Basidiocarp specimens of ectomycorrhizal basidiomycetes
were collected in the field and cultured in the laboratory by
basidiospore-drop and tissue culture methods. Attempts were
made to induce the germination of non-germinating basidiospores
with the addition of activated charcoal and a Rhodotorula
rubra colony to the Petri plates. Clitocybe nuda (Fr.)
Bigelow and Smith germinated rapidly and Laccaria laccata (Fr.)
Berk. and Br. germinated slowly without the supplements. A
few basidiospores of Tricholoma flavovirens (Fr.) Lund germinated
with the addition of activated charcoal. Mycelia of
Tricboloma flavovirens, Clitocybe nuda and Laccaria laccata
were inoculated onto plates containing autoclaved Pinus strobus
root segments, root segments surface-sterilized in 5% NaOCI,
and a root extract solution. The plates containing the surface-sterilized
root segments were all lost due to bacterial and
fungal contaminations. The fungi appeared to grow most
rapidly on the root extract solution.. The results obtained
from observing the response of the fungi to the autoclaved
root segments were inconclusive. The same three fungi were
grown in flasks containing single carbon sources to determine
the capability of the fungi to utilize different carbon
sources. The fungi were found to vary in their ability to
utilize the different carbon sources. Basidiocarps of
Laccaria laccata were obtained in some of the flasks.
Description
iv, 33 p.
Citation
Publisher
Kalamazoo College
License
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