Germination and Growth of Some Ectomycorrhizal Basidiomycetes in Culture
Losinger, Willard Carl
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Basidiocarp specimens of ectomycorrhizal basidiomycetes were collected in the field and cultured in the laboratory by basidiospore-drop and tissue culture methods. Attempts were made to induce the germination of non-germinating basidiospores with the addition of activated charcoal and a Rhodotorula rubra colony to the Petri plates. Clitocybe nuda (Fr.) Bigelow and Smith germinated rapidly and Laccaria laccata (Fr.) Berk. and Br. germinated slowly without the supplements. A few basidiospores of Tricholoma flavovirens (Fr.) Lund germinated with the addition of activated charcoal. Mycelia of Tricboloma flavovirens, Clitocybe nuda and Laccaria laccata were inoculated onto plates containing autoclaved Pinus strobus root segments, root segments surface-sterilized in 5% NaOCI, and a root extract solution. The plates containing the surface-sterilized root segments were all lost due to bacterial and fungal contaminations. The fungi appeared to grow most rapidly on the root extract solution.. The results obtained from observing the response of the fungi to the autoclaved root segments were inconclusive. The same three fungi were grown in flasks containing single carbon sources to determine the capability of the fungi to utilize different carbon sources. The fungi were found to vary in their ability to utilize the different carbon sources. Basidiocarps of Laccaria laccata were obtained in some of the flasks.