The Effect of Different Membrane Vesicle Preparation Techniques of Membrane Bound Enzymes of Paracoccus Denitrificans
Kellar, Daniel Philip
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Membrane vesicles from a single homogeneous culture af Paracoccus denitrificans were prepared in a variety of ways. This bacteria was converted into vesicles by means of sonic disruption, physical disruption using glass beads, and chemical disruption employing lysozyme. Each type of preparation exhibited some degree of inside - out orientation with respect to the physical make-up of the vesicle. Transhydrogenase, NADH - oxidase, ATPase and succinate dehydrogenase activities were found to be dependent upon the manner in which the vesicles had been prepared. Results are discussed in relative terms of their further applications in studies of specific enzymes.
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