A Comparison of M-1 Agar and Chocolate Agar for antibiotic Susceptibility for Testing ofNeisseria gonorrhoeae
Hyndman, Valerie A.
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This study was undertaken to determine if M-l agar, a simple synthetic medium used in genetic transformation studies of Neisseria gonorrhoeae, could also be used for antibiotic susceptibility testing, instead of the usual chocolate agar. Sixty strains of N. gonorrhoeae which had been isolated from patients were grown on duplicate plates containing M-l and chocolate agar. Each type of medium was tested with six antibiotics to which N. gonorrhoeae was susceptible. Thirty per cent of the strains did not grow sufficiently on M-l plates. Analysis of the zone sizes of strains showing growth on M-l and chocolate showed significant differences in zone sizes between agars except for ampicillin and penicillin. This suggests that the use of M-l agar might be useful only for penicillin and ampicillin. Further studies are necessary to determine the usefulness of M-l medium for other antibiotics.