Characterization of Hypoxic B16 Melanoma Cells
Hessburg, Thomas P.
MetadataShow full item record
Hypoxic cells (cells which are very low in oxygen content) are known to be present in large numbers in solid tumors. The hypoxic portion of a tumor is important in the treatment of cancer because it is less sensitive to radiation. The purpose of this project was to make B16 melanoma cells hypoxic and to examine their abilities to reproduce, grow, synthesize macromolecules and progress. through the cell cycle. The sensitivity of hypoxic cells to X-radiation and to mitomycin C (a drug which is known to be more toxic to hypoxic EMT6 cells) was also determined. Prolonged hypoxic conditions (48 hours) caused reproductive cell death, inhibited cell growth, inhibited the synthesis of macromolecules and caused a buildup of S phase cells. Cells which had been made hypoxic for four hours were less sensitive to X-rays (oxygen enhancement ratio is about 1.5) and showed about the same sensitivity to mitomycin C as oxic cells. Prolonged exposure to hypoxic conditions made the cells more susceptible to various· types of trauma such as X-rays and mitomycin C. •