Characterization of Hypoxic B16 Melanoma Cells
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Authors
Hessburg, Thomas P.
Issue Date
1980
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Hypoxic cells (cells which are very low in oxygen content) are known to be
present in large numbers in solid tumors. The hypoxic portion of a tumor is
important in the treatment of cancer because it is less sensitive to radiation.
The purpose of this project was to make B16 melanoma cells hypoxic and to
examine their abilities to reproduce, grow, synthesize macromolecules and progress.
through the cell cycle. The sensitivity of hypoxic cells to X-radiation and to
mitomycin C (a drug which is known to be more toxic to hypoxic EMT6 cells) was
also determined. Prolonged hypoxic conditions (48 hours) caused reproductive
cell death, inhibited cell growth, inhibited the synthesis of macromolecules
and caused a buildup of S phase cells. Cells which had been made hypoxic for
four hours were less sensitive to X-rays (oxygen enhancement ratio is about
1.5) and showed about the same sensitivity to mitomycin C as oxic cells.
Prolonged exposure to hypoxic conditions made the cells more susceptible to
various· types of trauma such as X-rays and mitomycin C.
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Description
iv, 42 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.