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dc.contributor.advisorDeutch, Charles E.
dc.contributor.authorFreedman, Leonard P.
dc.descriptionvi, 61 p.en_US
dc.description.abstractA mutant of Escherichia coli has been isolated which lacks leucyl, phenylalanyl-tRNA:protein transferase. The transferase catalyzes the addition of leucine, phenylalanine, or methionine to basic NH2-terminal residues of protein or peptide acceptors. It is reported here that the specific activities of the three enzymes coded for by the lactose operon are reduced in the mutant as compared to its parent and two spontaneous revertants, and that this reduction is most pronounced in cells grown with glucose as the carbon source. The results also indicate that when reduction occurs in the transferase-less mutant, percent activity of the lac operon enzymes progressively decreases, moving from the z gene (which codes for β -galactosidase) to the y gene (which codes for lac permease) to the a gene ( which codes for thiogalactoslde transacetylase). Because β-galactosidase and thiogalactoside transacetylase have been ruled out as potential substrates for the transferase, it is probable that the transferase acts on some protein involved in the overall control of transcription or translation of the lac operon. Mechanisms attempting to interpret the glucose and polarity effects in terms of a possible substrate for the transferase are discussed.en_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleReduced Lactose Operon Expression in an Escherichia Coli Mutant Lacking Leucyl, Phenylalanyl-tRNA: Protein Transferaseen_US
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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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