Structural Analysis of a Gene cluster Aspergillus nidulans
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A 13.3 kilobase pair (kbp) fragment of DNA from the fungus Aspergillus nidulans was isolated and ligated to the bacteriophage Charon 4A. The fragment contained structural genes homologous to poly(A)+ RNAs that are accumulated during conidiation and are not present in vegetative cells. Subfragments have now been isolated which, when nick translated and hybridized to Northern blots of developmental poly(A) + RNA, define the size, number and location of these structural genes.