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dc.contributor.advisorNeedleman, Richard Bruce
dc.contributor.authorDeWitt, Toby
dc.descriptionvi, 25 p.en_US
dc.description.abstractThis report presents the results of three experiments on a recombinant plasmid called the ilv 1 plasmid. This plasmid carries the ilv 1 gene of Saccharomyces cerevisiae which produces Threonine deaminase. In the first experiment the presence of this plasmid in a strain of S. cerevisiae called 3971 (i1v 1) is proven by the observation that 50% of the cells in a given clone will segregate the ilv 1 plasmid. The second experiment involved the determination of the specific activity of Threonine deaminase in strain 3971 (i1 v 1) and a wild type strain, CBII, after growing the cells in repressing (+I1e) and non-repressing (-lIe) media. The results show that the basal level of Threonine daminase in 3971 (i1v 1) is four-fold that of the control, CBll. The specific activity of Threonine dearninase in 3971 (i1v 1) cultured non-repressing medium is I5-fold the specific activity of Threonine daminase in repressing medium. The result however does not prove that the ilv 1 gene is being regulated by lIe because only 2% of the 3971 (ilv 1) cells in the repressing medium were carrying the plasmid at the time of harvest. In the third experiment a strain, D160-2C, of S. cerevisiae having an arg 6 nonsense mutation was transformed with the i1v 1 plasmid. It was found that 80% more transformed than untransforrned colonies grew on media without Arg, indicating the presence of the gene on the i1v I plasmid.en_US
dc.description.sponsorshipDepartment of Biochemistry. Medical School. Wayne State University. Detroit, Michigan.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titlePreliminary Experiements on the ilv 1 Gene of Saccaromyces Cerevisiae, Located on a Plasmid Recombined from a Yeast Plasmid, An e. Coli Plasmid and an Insert of Yeast Chromosomal DNAen_US
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  • Biology Senior Individualized Projects [1520]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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