A Partial Characterization of Ion Transport in the Isolated Toad Cornea
Zimmerman, Warren Brett
MetadataShow full item record
The cornea has been shown to be a suitable model of the.ascending limb of the loop of Henle. Cl- transport in toad cornea can be quantitated by short-circuit current (SCC) techniques. This method was employed to study the effects of experimental agents on C1- transport in the cornea. It is hoped that characterization of this transport will help in the design of a new and more effective loop diuretic. In the present study, it was observed that; (a) toad corneas were found to react with indanone in much the same fashion as they react to ethacrynic acid and its cysteine adduct, (b) metabolic inhibitors, such as 2,4-dinitrophenol, did not clearly distinguish non-selective metabolic effects from the suspected selective effects obtained with loop diuretics, (c) amphotericin B lead to results which will require a further investigation of its effects, and (d) the results with the known diuretics lethacrynic acid and its cysteine adduct) were consistent with a recently proposed model (Frizzell et a1.,1979) of transepithelial chloride transport.