A Partial Characterization of Ion Transport in the Isolated Toad Cornea
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Authors
Zimmerman, Warren Brett
Issue Date
1979
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The cornea has been shown to be a suitable model of the.ascending limb
of the loop of Henle. Cl- transport in toad cornea can be quantitated by
short-circuit current (SCC) techniques. This method was employed to study the
effects of experimental agents on C1- transport in the cornea. It is hoped that
characterization of this transport will help in the design of a new and more
effective loop diuretic. In the present study, it was observed that; (a) toad
corneas were found to react with indanone in much the same fashion as they react
to ethacrynic acid and its cysteine adduct, (b) metabolic inhibitors, such as
2,4-dinitrophenol, did not clearly distinguish non-selective metabolic effects
from the suspected selective effects obtained with loop diuretics, (c) amphotericin
B lead to results which will require a further investigation of its effects,
and (d) the results with the known diuretics lethacrynic acid and its cysteine
adduct) were consistent with a recently proposed model (Frizzell et a1.,1979)
of transepithelial chloride transport.
Description
v, 23 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.