An Investigation into the Resistance Development of Carcinogenic B16 Melanoma Cells Treated with 7-Con-O-Methylogarol (7-Omen)
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An important factor in cancer research today is the development of cell resistance to chemotheraputic drugs. Resistance begins with some form of genetic alteration that allows the carcinogenic cell to become unaffected by the destructive power of the drug. Researchers today are investigating ways in which this drug resistance develops. One form of investigation being done is to expose known carcinogenic cell lines in vitro to chemotheraputic drugs. This project dealt specifically with the exposure in vitro of the cell line B-16 Melanoma (skin cancer) cells to a new anthracycline drug, 7-Con-O-Methylnogarol (7-Omen). Previous experiments utilizing similar techniques and cell lines with other anthracycline drugs determined that in vitro drug resistance would form after drug exposure and subculturing. The main purpose of this experiment was to develop cell resistance to the drug 7-Omen. The basic technique followed in this experimentation consisted of culturing (attaching and growing) B-16 cells in plastic tissue culture flasks, passaging the cells and then exposing the cells to the drug 7-Omen through addition of the drug to the cell culture medium. Drug contact with the cells was allowed for a predetermined finite (2 hours, 4 hours,etc.) or continuous time period. Results of such experiments were then compared to the growth curves of normal untreated B-16 cells and conclusions determined. Also done were observations and photographic displays of the gross morphological changes exhibited by the cells after drug exposure.