Two-Dimensional Gel Electrophoretic Analysis of Urinary Proteins in the Sprague-Dawley Rat
Jones, Donald H.
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Proteinuria is a symptom common to male Sprague-Dawley rats with chronic progressive nephrosis. Albumin and other high molecular weight proteins are excreted in the urine as rats age. In these experiments urine, serum, kidney cortex, medulla, glomeruli, and glomerular basement membrane samples of juvenile (2 months), retired breeder (7 months), and aged adult rats (26 months) were compared by two-dimensional gel electrophoresis. Proteins were separated according to their isoelectric point and molecular weight, and stained by a silver nitrate procedure. A low molecular weight protein (17,000 daltons) was repeatedly found in urine samples of juvenile and retired breeder rats. The protein appeared to be derived from the cortex, since it was absent in samples from the medulla, glomeruli, and glomerular basement membrane. This protein may serve as a diagnostic marker for younger rats that do not yet exhibit the symptoms of chronic progressive nephrosis.