Dietary Effects on Protein Expression in the Prediabetic Chinese Hamster
Raedy, Melany Fairbanks
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Diet plays an important role in the expression of diabetes mellitus and dietary manipulation can result in amelioration of hyperglycemia and glycosuria. The molecular mechanism of the amelioration is unknown; however, the mode of action presumably reflects permanent changes in the diabetic's nuclear gene expression. To determine the relationship between diet and severity of diabetes and changes in nuclear proteins, prediabetic Chinese hamsters were fed diets differing in fat content. These animals were tested for severity of diabetes, their hepatic nuclei were isolated and the nuclear proteins were separated on two-dimensional gel electrophoretograms. The results confirm previous studies that low fat diet ameliorates diabetes in the Chinese hamster model, establish the feasibility of the experimental approach, and present evidence that the quantity of at least one group of nuclear proteins seems to be reduced in most low fat fed hamsters.