Preliminary Investigation of Actin Gene Organization in Sea Urchin Species, Lytechinus pictus
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Starting with a genomic library of DNA from the sea urchin, Lytechinus nictus, restriction maps were formed of three recombinant clones, LPA 31, LPA. 32, & LPA 81. The objective of this study was to begin preliminary investigation of gene multiplicity and organization of the actin gene family in this species. This research was carried out as part of a project involving investigation of actin's organization in three species of sea urchin, Stronglyocentratus purpuratus, Stronglyocentratus franciscanus, and Lytechinus pictus. Using various restriction enzymes, DNA from L.pictus was cleaved and the ensuing fragments were separated and sized on Agarose gels. Fragments were then transferred to nitrocellulose filters using the Southern blot technique, and hybridized with a radioactively-labelled actin cDNA probe to reveal actin coding sequence. Actin coding sequence has been shown to span 4.8 kb, 2.2kb, and 3.4 kb in LPA 31, 32, and 81 respectively. These results suggest the existence of at least two pairs of linked actin genes in this species.