The Effects of Mercuric Chloride, A23187, and CC-1065 on Primary Adult Rat Hepatocyte Cultures
Chase, Robin L.
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Recent developments in the culture of primary rat hepatocytes have produced a tool for in vitro toxicity studies. Consequently, the purpose of this study is to establish a basis as to how these cultures respond to various toxins and how best to evaluate these responses. Three agents thought to have differing mechanisms of toxicity were chosen; mercuric chloride (HgC1 2); A23187, a divalent ionohore; and CC-I065, an experimental anti-tumor agent. Cytotoxoic effects were evaluated by trypan blue exclusion, and by the release of organelle-membrane-bound serum glutamic oxaloacetate transaminase (SGOT), and cytoplasmic lactate dehydrogenase (LDH). Our findings suggest that trypan blue exclusion is a consistent indicator of cytotoxicity, whereas SGOT and LDH release are not as reliable, depending on the toxin.