Secretion of Ascorbate from Isolated Bovine Adrenal Medullary Cells
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Authors
Asher, Anthony L.
Issue Date
1982
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Ascorbate has been found in very high concentration in chromaffin
cells and secretory vesicles of the adrenal medulla. It was hypothesized
that this ascorbate may be secreted concomitantly with catecholamines.
Three (3) day old cultured bovine chromaffin cells were
prelabelled with 14C - ascorbate for several hours and induced to release
with veratradine. It was found that approximately 30% of the
label was secreted upon veratradine stimulation and that such secretion
was inhibited by tetrodotoxin. Secretion was not, however, inhibited
by isethionate or absence of calcium. This data is taken to
indicate that ascorbic acid is released from chromaffin cells and that
this release is primarily cytoplasmic.
Description
iii, 43 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.