The Effects of Antibiotics on Leukocyte Chemotaxis
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Twenty three antibiotics, possessing a wide variety of biochemical modes of action, were surveyed for their effects on human leukocyte chemotaxis in vitro. Chemotaxis was measured using a synthetic tripeptide and filtrates from cultures of E. coli and P. granulosum. Most of the antibiotics had no specific inhibitory effects. However, consistent chemotactic inhibition was seen with those antibiotics whose antibacterial mode of action is inhibition of RNA synthesis. These included: tirandamycin, rifamycin B, streptolydigin, streptovaricin, and rifampicin. The inhibitory effect of one antibiotic, rifampicin, was found to be a function of the concentration of the chemotactic agent; that is, high concentrations of rifampicin, which were inhibitory at low chemotactic agent concentrations, not only were no longer inhibitory, but they appeared to actually reverse the deactivation of chemotaxis caused by excessively high. concentrations of chemotactic agents.