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dc.contributor.advisorMarshall, V. P.
dc.contributor.authorSlavicek, James M.
dc.descriptionii, 37 p.en_US
dc.description.abstractChaetomium sp: UC®-4634 catalyzes ketonic carbonyl reduction of steffimycin B to 10-dihydrosteffimycin B (Scheme 1). Using cell-free preparation of Chaetomium sp. UC®-4634, the process of ketonic carbonyl reduction has been shown to be TPNH linked and dependent upon the age of the Chaetomium culture used. The cell-free extract from Chaetomium had the highest enzymatic activity when harvested after 48 hours of growth. The reaction rate increased linearly with added crude enzyme protein up to 1.6 mg/ml and was highest at approximately pH 6.0. The . . conversion of steffimycin B to 10-dihydrosteffimycinB was found to be inhibited by potassium phosphate. Chaetomium s.p. UC-4634 was also found to catalyze the ketonic carbonyl reduction of steffimycin to 10-dihydrosteffimycin. The products were isolated by extraction from the reaction mixtures and identified by comparison of their Rf values with those of authentic materials.en_US
dc.description.sponsorshipUpjohn Company
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleThe Characterization of the Enzymatic Conversion of Steffimycin B to 10-Dihydrosteffimycin Ben_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1457]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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