The Characterization of the Enzymatic Conversion of Steffimycin B to 10-Dihydrosteffimycin B
Loading...
Authors
Slavicek, James M.
Issue Date
1978
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Chaetomium sp: UC®-4634 catalyzes ketonic carbonyl reduction of
steffimycin B to 10-dihydrosteffimycin B (Scheme 1). Using cell-free
preparation of Chaetomium sp. UC®-4634, the process of ketonic carbonyl
reduction has been shown to be TPNH linked and dependent upon the age
of the Chaetomium culture used. The cell-free extract from Chaetomium
had the highest enzymatic activity when harvested after 48 hours of
growth. The reaction rate increased linearly with added crude enzyme
protein up to 1.6 mg/ml and was highest at approximately pH 6.0. The . .
conversion of steffimycin B to 10-dihydrosteffimycinB was found to be
inhibited by potassium phosphate. Chaetomium s.p. UC-4634 was also
found to catalyze the ketonic carbonyl reduction of steffimycin to
10-dihydrosteffimycin. The products were isolated by extraction from
the reaction mixtures and identified by comparison of their Rf values
with those of authentic materials.
Description
ii, 37 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.