Restriction Analysis of Two Escherichia Coli Hybrid Plasmids
The development of antibiotic resistant microorganisms has been a serious problem in the clinical use of antibiotics. For example, the introduction of sulfonamides (SA) for the treatment of bacillary dysentery in Japan following World War II at first proved extremely effective and the number of cases of dysentery declined dramatically. However, in 1949, sulfonamide-resistant strains of Shigella, the bacterium which causes dysentery, began to appear. Despite continued usage of these drugs, the number of reported cases of dysentery has climbed. The introduction of new antibiotics such as tetracycline (Tc), chloramphenicol (Cm), and streptomycin (Sm) alleviated the problem for a few years. However, strains resistant to these drugs are now frequently isolated.