Plant Propagation of Salt Marsh Species Through Aseptic Culture Techniques
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Authors
Musselman, Mary R.
Issue Date
1977
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
An investigation was carried out to evaluate media,
plant parts and techniques involved in the propagation of
various species of monocotyledonous plants through tissue
culture methods. All but one species used were salt
marsh plants. These were Spartina alterniflora, Spartina
patens, Distichlus spicata, and Juncus roemerianus.
Uniola paniculata, a sand dunes species, was the other
plant used. Excised shoot and root tips and lateral buds of
S. alterniflora were implanted in nutrient media. Half of
the media were of known chemical composition. The other
half were prepared with filtered sea water and the exact
inorganic composition was unknown. The relative cytokinin
and auxin levels of the media was varied. No growth occurred
in any of the cultures of S. alterniflora. Contamination
was minimal in these cultures. Excised lateral buds of
different sizes obtained from the six species were implanted
on Murashige and Skoog Shoot Multiplication Medium B.
There was shoot elongation in some cultures of each species
except S. alterniflora. The amount of contamination varied
with the different sizes of buds and seemed to vary between
species. Growth of the plants had just begun when the
project ended. More work must be done with these plants
before their actual propagation through tissue culture can
be realized.
Description
ii, 23 p.
Citation
Publisher
Kalamazoo College
License
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