Plant Propagation of Salt Marsh Species Through Aseptic Culture Techniques
Musselman, Mary R.
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An investigation was carried out to evaluate media, plant parts and techniques involved in the propagation of various species of monocotyledonous plants through tissue culture methods. All but one species used were salt marsh plants. These were Spartina alterniflora, Spartina patens, Distichlus spicata, and Juncus roemerianus. Uniola paniculata, a sand dunes species, was the other plant used. Excised shoot and root tips and lateral buds of S. alterniflora were implanted in nutrient media. Half of the media were of known chemical composition. The other half were prepared with filtered sea water and the exact inorganic composition was unknown. The relative cytokinin and auxin levels of the media was varied. No growth occurred in any of the cultures of S. alterniflora. Contamination was minimal in these cultures. Excised lateral buds of different sizes obtained from the six species were implanted on Murashige and Skoog Shoot Multiplication Medium B. There was shoot elongation in some cultures of each species except S. alterniflora. The amount of contamination varied with the different sizes of buds and seemed to vary between species. Growth of the plants had just begun when the project ended. More work must be done with these plants before their actual propagation through tissue culture can be realized.