Two Experimental Systems for the Study of Amino Acid Transport in Saccharomyces Cerevisiae
Kreisle, Regina A.
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The chemiosmotic coupling hypothesis has been proposed as a model for the coupling of energy metabolism with amino acid transport in S. cerevisiae. Although this model has been extensively studied in procaryotic systems, it has not been well characterized in eucaryotic systems. Isolation of a mutant yeast strain with altered plasma membrane ATPase activity and the preparation of spheroplasts may serve as possible experimental systems for the study of this model in yeast.