A Comparison of Dimethyl Sulfoxide and Butyric Acid as Inducers of Hemoglobin Synthesis in Friend Leukemia Cells
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Authors
Stine, Oscar Colin
Issue Date
1976
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
A comparison was made of butyric acid (BA) and dimethyl sulfoxide
(DMSO) as inducers of differentiation in the Friend Leukemia Cells
CM86 #745. Stimulation of hemoglobin production was found to be both
quantitatively and qualitatively different. Neither BA nor DMSO
induced stationery phase cells to undergo differentiation. Thymidine
was used to demonstrate that BA does not need two cell doublings
before it induces hemoglobin production in the cells. BA and DMSO
produce the same types of hemoglobin as determined by electrophoresis.
When passage 10 (P.10) cells were used instead of passage 190 (P.l90),
BA was found not to induce the cells. DMS0 did induce the P.10
cells but to a lesser extent than it did with the P.l90 cells.
Hemoglobin antibody and [3H]-Leucine were used in an attempt
to take a closer look at the kinetics of an induction by both
BA and DMSO. A highly unusual agar diffusion pattern was discovered
and partially explained. Two models are proposed. One has a fatal flaw in it. The other
explains much of evidence found in earlier literature. This supporting
evidence is presented.
Description
iv, 25 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.