A Comparison of Dimethyl Sulfoxide and Butyric Acid as Inducers of Hemoglobin Synthesis in Friend Leukemia Cells
Stine, Oscar Colin
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A comparison was made of butyric acid (BA) and dimethyl sulfoxide (DMSO) as inducers of differentiation in the Friend Leukemia Cells CM86 #745. Stimulation of hemoglobin production was found to be both quantitatively and qualitatively different. Neither BA nor DMSO induced stationery phase cells to undergo differentiation. Thymidine was used to demonstrate that BA does not need two cell doublings before it induces hemoglobin production in the cells. BA and DMSO produce the same types of hemoglobin as determined by electrophoresis. When passage 10 (P.10) cells were used instead of passage 190 (P.l90), BA was found not to induce the cells. DMS0 did induce the P.10 cells but to a lesser extent than it did with the P.l90 cells. Hemoglobin antibody and [3H]-Leucine were used in an attempt to take a closer look at the kinetics of an induction by both BA and DMSO. A highly unusual agar diffusion pattern was discovered and partially explained. Two models are proposed. One has a fatal flaw in it. The other explains much of evidence found in earlier literature. This supporting evidence is presented.