In Vivo Nitrite Accumulation as an Indicator of Herbicide Activity
Spaller, Donna M.
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In vivo nitrite accumulation was studied in several plants with experimental compounds and standard herbicides to determine the effectiveness of a new assay method for detecting potential herbicides. The mechanism involved blockage by compound, of electron flow necessary for nitrite reduction; nitrite is a toxic form of nitrogen in plants. The method effectively detected photosynthetic inhibitors but was not effective for compounds having other mechanisms of action. Factors within the assay which had a significant influence on nitrite accumulation and/or nitrate reductase levels within the tissue were: age of the tissue, degree of light pretreatment, concentration of compound, length of incubation interval, species of plant, and type of surfactant. Formulation of compound did not seem to effect the response in this assay.