Study of Drug Effects on Non-Transformed and Transformed Mammalian Cells in Culture
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Authors
Ludington, Lana
Issue Date
1976
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
This investigation was undertaken to study the
feasibility of using both transformed and non-transformed
cell lines as a preliminary screening system for identifying
antitumor agents. Agents selected from this testing
system should show preferential toxicity towards the highly
tumorigenic transformed cells.
The three cell lines used in this study were nontransformed
BALB 3T3 (A-31) and the transformed cell lines,
KA31 and R4 (both subclones of BALB 3T3 cells). The
cytotoxicity of several classes of clinically useful anticancer
drugs towards these cell lines was determined by
both inhibition of cell growth and cell-kill (cloning assay).
In the growth inhibition assay only cytosine arabinoside
and 6-mercaptopurine showed a marked inhibitory effect
of KA31 and R4 cells as compared to the BALB 3T3 cells.
Cytosine arabinoside also showed the differential effect
towards the two transformed cell lines in the cell-kill
assay. None of the other anticancer drugs tested showed
this differential cytotoxicity. These results as related
to the purpose of this investigation were discussed.
Description
v, 42 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.