The Isolation and culture of Haploid Protoplasts from the African Violet and Kohleria (family Gesnariaceae)
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Authors
Covert, Nancy L.
Issue Date
1976
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Protoplasts, single plant cells without their cell walls,
have been isolated and, in some cases, induced to regenerate
to adult plants in various species, though never before in the
African violet and rarely with haploid plants. Uses of protoplasts
include somatic cell fusion, macromolecule and organelle
uptake, and studies of the plasmalemma and cell wall biosynthesis.
The use of haploids introduces further possibilities
in genetic studies and mutation. In this study protoplasts
and single cells were isolated by enzymic digestion from greenhouse
grown haploid plants and cultured haploid plantlets
(African violet1 and from cultured callus tissue (Kohleria).
Cell Viability, survival, and cell wall regeneration were observed
as both isolation techniques and culture conditions
were examined to optimize cell production and survival, and
to induce cell division.
Description
iv, 47 p.
Citation
Publisher
Kalamazoo College
License
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