The Isolation and culture of Haploid Protoplasts from the African Violet and Kohleria (family Gesnariaceae)
Covert, Nancy L.
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Protoplasts, single plant cells without their cell walls, have been isolated and, in some cases, induced to regenerate to adult plants in various species, though never before in the African violet and rarely with haploid plants. Uses of protoplasts include somatic cell fusion, macromolecule and organelle uptake, and studies of the plasmalemma and cell wall biosynthesis. The use of haploids introduces further possibilities in genetic studies and mutation. In this study protoplasts and single cells were isolated by enzymic digestion from greenhouse grown haploid plants and cultured haploid plantlets (African violet1 and from cultured callus tissue (Kohleria). Cell Viability, survival, and cell wall regeneration were observed as both isolation techniques and culture conditions were examined to optimize cell production and survival, and to induce cell division.