A Study of Phytohemagglutinins : an Investigation of an a-D-Galactose Binding Lectin
Ross, Timothy Todd
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The B. simplicifolia lectin directly coupled to cyanogen bromide-activated Sepharose 4B has been shown an effective tool to reaffirm this lectin's carbohydrate binding specificity. Study of this lectin's interaction with three classes of compounds; polysacchari.des, carbohydrateprotein conjugates, and sugar nucleotides have allowed the close examination of lectin-carbohydrate interactions. The described affinity chromatography system offers new opportunities for the isolation of a-D-galactose containing biomolecules. Value lies not only in selective purification, but in characterizing the sometimes subtle chemical differences of biomolecules. The unique and intriguing biological and chemical properties of' lectins make this class of compounds important in future investigation of intercellular and cell surface phenomena.