A Study of the Growth of Respiratory Syncytial Virus in Irradiated Hela Cells
MetadataShow full item record
Investigations were conducted to test whether Respiratory Syncytial(RS) virus would replicate in tissue cultures of HeLa cells that had been exposed to a dose of X-irradiation that inhibited cell division. Cells were seeded in plastic petri dishs and exposed to X-rays, then infected with RS virus at different times after irradiation; yields af virus were assayed by the plaque method. It was found that there was no significant difference in yields of RS virus from irradiated and non-irradiated tissue cultures, and that the time of infection (up to 6 days after exposure) did not significantly affect virus replication. This study may provide a system in which virus synthesis can proceed in cells whose biosynthetic activity has been reduced from the level that occurs under normal conditions, i.e., in non-irradiated cells.